Please use this identifier to cite or link to this item: http://theses.ncl.ac.uk/jspui/handle/10443/4918
Title: Ammonium Signalling in Dimorphic Fungi
Authors: Lister, Siobhan Hope
Issue Date: 2020
Publisher: Newcastle University
Abstract: Ammonium is a preferred source of nitrogen utilised by fungi. In some fungi ammonium availability is sensed by ammonium transporters belonging to the Amt/Mep/Rh superfamily. During ammonium limiting conditions these transporters trigger a signalling cascade to induce a morphological change. The molecular basis for signalling and the extent to which these transporters are conserved are important questions within the field. We have investigated morphological change in response to ammonium availability in two divergent fungi. The wheat pathogen Zymoseptoria tritici and the human pathogen Cryptococcus neoformans serotype D JEC20 (MATa) and JEC21 (MATa). We show that low ammonium dependent filamentation is ZtMep2 independent and that mutants lacking ZtMep3 acquire a severe growth defect during ammonium sufficient conditions. Moreover, Ztmep3D mutants display a different type of filamentation which may be as a result of ammonium starvation as opposed to ammonium signalling. ZtMep3 does however act as an ammonium sensor when expressed in yeast, to regulate pseudohyphal growth, despite lacking the conserved twin-histidine motif previously believed to be essential for signalling. Furthermore, the dual loss of ZtMep2 and ZtMep3 renders Z. tritici hypervirulent in the wheat infection assay suggesting that a lack of internal ammonium is the trigger for virulence. In C. neoformans, we show that hyphal growth, induced during ammonium limiting conditions, is CnAmt2 dependent and that the expression of the CnAmt2N241A mutant in yeast uncouples transport from signalling. Therefore, signalling by CnAmt2 is not the consequence of internal ammonium metabolism and is due to the physical act of transport. Fundamental questions now exist as to why these two diverse fungi have adopted different modes of ammonium signalling and about how prevalent these starvation responses are throughout fungi.
Description: Ph. D. Thesis
URI: http://theses.ncl.ac.uk/jspui/handle/10443/4918
Appears in Collections:Institute for Cell and Molecular Biosciences

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